Western Blotting

• Blotting Membranes, Sandwiches, Filter Papers
• Chemiluminescent Reagents

 

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Immobilon Transfer Membranes, Sandwiches, and Blotting Filter Paper

Polyvinylidene fluoride (PVDF) for superior western, dot, and slot blots
Millipore offers three different Immobilon PVDF membranes, each optimized for different protein blotting applications. We also now offer blotting sandwiches that feature pre-cut sheets of membrane and blotting filter paper.

Immobilon membranes provide a number of advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

Immobilon-P Membrane
- 0.45 µm pore size
- Recommended for most western blotting, especially proteins >20 kDa
- Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

Blotting Sandwiches
- When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper.

Blotting Filter Paper
- Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

Immobilon-P^SQ Membrane
- 0.2 µm pore size and a large internal structure
- Higher protein adsorption and sequencing yields than other membranes
- Recommended for blotting proteins <20 kDa
- Prevents blow-through of low molecular weight proteins

Immobilon-FL Membrane
- The first transfer membrane optimized for fluorescence applications
- Extremely low background improves sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
- Ideal for multiplexing and chemifluorescence applications

Immobilon Western Chemiluminescent HRP Substrate

High sensitivity over a broad detection range
Immobilon Western HRP Chemi luminescent Substrate provides high sensitivity over a broad detection range at a lower cost than other HRP substrates.

This combination of versatility and value makes Immobilon Western HRP substrate the only HRP reagent most labs will need for all of their western blotting experiments.

Reduces Antibody Consumption
Immobilon Western Substrates can lower antibody consumption by 2 to 5 fold compared to less sensitive reagents.

More Sensitive
The substrate's high signal intensity and low background allow detection of high and low abundance proteins.

Versatile
Immobilon Western Substrates are compatible with both PVDF, nitrocellulose, and all commonly used buffers and blocking reagents.

Chemiluminescent Reagents

Immobilon Western AP Chemiluminescent Substrate

A high sensitivity, long lasting substrate
Immobilon Western AP Substrate is an enhanced, ready-to-use dioxetane derivative that provides higher sensitivity than other AP substrates at a lower price.

The substrate’s high signal intensity, long signal duration, and low background make it the most versatile AP substrate. It is the only AP reagent most labs need for all of their western blotting experiments.

Reduces Antibody Consumption
Immobilon Western Substrates can lower antibody consumption by 2 to 5 fold compared to less sensitive reagents.

More Sensitive
The substrate's high signal intensity and low background allow detection of high and low abundance proteins.

\Versatile
Immobilon Western Substrates are compatible with both PVDF, nitrocellulose, and all commonly used buffers and blocking reagents.